GTAC@MGI Sample Delivery Instructions

Prior to delivering your samples, please complete your Service Request here. Please include a copy of the Service Request with the samples.

Hours for delivery: 8AM – 4PM
Monday – Friday
4444 Forest Park Avenue, 4th Floor
Phone is available in office area northeast of elevators if needed

Illumina and Sample Quality Control

Total RNA and ChIP samples

• Room 4125: Located to the left of the wall mural
• Please call 314-362-1549 when you arrive and we will meet you at the door
• Please do not leave RNA samples in the 4°C Drop Off Fridge in the reception area

Prepared Libraries and DNA

• 4°C Drop Off Fridge: Located on 4th floor, follow signs to the McDonnell Genome Institute office
• Please deliver samples in a secondary container (bag or box) labeled with Lab Name and Service Request number to the appropriate container:
• DNA: green bin on the bottom shelf labeled “Research – DNA Only”.
• Libraries:
– Blue bin on the bottom shelf labeled “Research – Libraries qPCR & Sequencing Only”
– Black bin on the bottom shelf labeled “Research – Libraries for Final Library QC”
– Yellow Bin on the bottom shelf labeled “Research – Libraries for 10x Library QC”
• Notify the GTAC@MGI that samples have been dropped off: mgi-sample@gowustl.onmicrosoft.com

Single-cell/single-nuclei suspensions or cDNA for 10x Genomics library prep and sequencing

• Date and time of delivery must be scheduled with the 10x Single Cell Core prior to sample delivery.

• Schedule online at https://book.appointment-plus.com/d5jybymq/
• We are available from 10am-4:00pm Monday-Friday excluding holidays.
• Additional QC of the cells will NOT be performed at the GTAC@MGI, concentration must be determined by the lab providing the samples.
• For a scheduled delivery, please come to the 4th floor and call 314-286-1957. Someone from the team will then meet you by the elevators and bring you to the lab for sample transfer.
• Cells must be delivered on ice and will be processed immediately upon receipt.

• Questions? Please contact the following.

• Jen Ponce (jgodfrey@wustl.edu) – Single Cell Manager
• GTAC@MGI 10x team mgi-10x-samples@gowustl.onmicrosoft.com

PacBio, Bionano, and Oxford Nanopore

Specimens, DNA, Total RNA, cDNA and Prepared Libraries

• Drop off date/time will be prearranged with Chris Markovic (cmarkovi@wustl.edu) during project discussion
• Will meet at 4th floor elevators for sample drop off
• Please call 314-286-1463 if there is no one waiting at elevators when you arrive

• Please do not leave samples in the 4°C Drop Off Fridge in the reception area

PCR, Fluidigm, and 16s

• Drop off date/time will be prearranged with Chris Sawyer (csawyer@wustl.edu)
• Drop off at Room 4117A: Located on 4th floor south hallway past the water fountain

• Please call 314-362-0059 if no one hears the door bell
• Please do not leave samples in the 4°C Drop Off Fridge in the reception area

Somalogics

• Drop off date/time will be prearranged with Andrew Lutz (alutz@wustl.edu)

• Will meet at 4th floor elevators for sample drop off
• Please call 314-273-5294 if there is no one waiting at elevators when you arrive
• Please do not leave samples in the 4°C Drop Off Fridge in the reception area

Specimens for Extractions

• Drop off date/time will be prearranged with Chris Sawyer (csawyer@wustl.edu)
• Drop off at Room 4117A: Located on 4th floor south hallway past the water fountain
• Please call 314-362-0059 if no one hears the door bell
• Please do not leave samples in the 4°C Drop Off Fridge in the reception area unless you have arranged times in advance and alert the team when the samples have been dropped off.  The yellow bin is not checked regularly.