Product & Service Details

NovaSeq2

About Illumina Sequencing

GTAC@MGI was an initial leader in sequencing for multiple iterations of the Illumina technology. Next-Generation Sequencing captures the process of massively parallel sequencing of billions of fragments simultaneously per Illumina run. This high-throughput process can translate into sequencing dozens of human genomes in a single run.

The Genome Access Technology Center can assist you with any of these instrumentation options.

NovaSeq 6000

  •  SP with 100, 200, 300, or 500 cycles
  •  S1 with 100,  200, or 300 cycles
  •  S2 with 100, 200, or 300 cycles
  •  S4 with 200 or 300 cycles

MiSeq

  • Version V2  300 or 500 cycles
  • Version V3  600 cycles

In addition to these standard flowcells and sequencing kits, GTAC@MGI can potentially support other Illumina sequencing options.  Please inquire.

What types of Sequencing are available?

Whole Genome Sequencing

A method used to analyze the entire genomic sequence of an organism that has been previously classified. We use a PCR-free method that can handle low DNA input for our automated high throughput process.

Whole Exome & Targeted Sequencing

Whole Exome is a method used to analyze the protein-coding regions of genes in the genome. Targeted sequencing includes an enrichment that focuses on specific genes or gene regions of interest.

  • IDT Whole Exome Capture
    • We work with DNA and cDNA as a Single Hyb Capture. This can accommodate up to 16 samples per hyb capture depending on the coverage metrics desired. Analyzing for germline variants will commonly utilize a targeted coverage of 50X, 75X, or 100X. Somatic analysis often targets 100X,150X, or 200X coverage.
  • IDT Custom Capture
    • We will work with the vendor and you to design a custom panel. Single hybridization captures can accommodate 1-96 samples depending on the genomic region being targeted.

Bacterial 16S Sequencing

The identification, classification, and quantitation of bacteria microbes at a species level through sequencing the 16S gene for both conserved and variable regions.

Options for 16S Illumina Sequencing  8 hypervariable regions with the use of the Fluidigm for library preparation and  MVRSION analysis for multiple hypervariable region analysis.

ChIPseq

A method to analyze protein interactions with DNA by combining chromatin immunoprecipitation(ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.  

Amplicon-Seq

Ultra deep sequencing of PCR products for known regions of interest to analyze variations in the genome. Bacterial 16S sequencing is a type amplicon sequencing. This highly targeted approach enables a wide range of applications for discovering, validating, and screening genetic variants in a rapid and efficient manner.

Direct to Sequencing

Please contact us for options to generate libraries in your lab and sequence in ours.  GTAC@MGI can include partial runs on a NovaSeq 6000 s4 to accommodate your project.

Library Preparation Options

For each sequencing option we have corresponding library preparation choices. 

Whole Genome

  • KAPA Hyper PCR-Free  with an automated process
  • SWIFT with a manual process and low input to start.
  • SWIFT WGBS  with a manual process and low input to start.
  • IDT with Unique Molecular Identifiers (UMIs) and a manual process with low input to start.

The above options include initial DNA quantitation check with the Qubit.

Whole Exome

  • KAPA Hyper amplified with an automated process

The above option includes initial DNA quantitation check with the Qubit.

ChIP-seq

This preparation includes initial DNA quality and quantitation check with the Qubit and Tapestation or the Bioanalyzer.