Product & Service Details


About Illumina Sequencing

GTAC@MGI was an initial leader in sequencing for multiple iterations of the Illumina technology. Next-Generation Sequencing captures the process of massively parallel sequencing of billions of fragments simultaneously per Illumina run. This high-throughput process can translate into sequencing dozens of human genomes in a single run.

The Genome Access Technology Center can assist you with any of these instrumentation options.

NovaSeq 6000

  •  SP with 100, 200, 300, or 500 cycles
  •  S1 with 100,  200, or 300 cycles
  •  S2 with 100, 200, or 300 cycles
  •  S4 with 200 or 300 cycles


  • Version V2  300 or 500 cycles
  • Version V3  600 cycles

In addition to these standard flowcells and sequencing kits, GTAC@MGI can potentially support other Illumina sequencing options.  Please inquire.

What types of Sequencing are available?

Whole Genome Sequencing

A method used to analyze the entire genomic sequence of an organism that has been previously classified. We use a PCR-free method that can handle low DNA input for our automated high throughput process.

Whole Exome & Targeted Sequencing

Whole Exome is a method used to analyze the protein-coding regions of genes in the genome. Targeted sequencing includes an enrichment that focuses on specific genes or gene regions of interest.

  • IDT Whole Exome Capture
    • We work with DNA and cDNA as a Single Hyb Capture. This can accommodate up to 16 samples per hyb capture depending on the coverage metrics desired. Analyzing for germline variants will commonly utilize a targeted coverage of 50X, 75X, or 100X. Somatic analysis often targets 100X,150X, or 200X coverage.
  • IDT Custom Capture
    • We will work with the vendor and you to design a custom panel. Single hybridization captures can accommodate 1-96 samples depending on the genomic region being targeted.

Bacterial 16S Sequencing

The identification, classification, and quantitation of bacteria microbes at a species level through sequencing the 16S gene for both conserved and variable regions.

Options for 16S Illumina Sequencing  8 hypervariable regions with the use of the Fluidigm for library preparation and  MVRSION analysis for multiple hypervariable region analysis.


A method to analyze protein interactions with DNA by combining chromatin immunoprecipitation(ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.  


Ultra deep sequencing of PCR products for known regions of interest to analyze variations in the genome. Bacterial 16S sequencing is a type amplicon sequencing. This highly targeted approach enables a wide range of applications for discovering, validating, and screening genetic variants in a rapid and efficient manner.

Direct to Sequencing

Please contact us for options to generate libraries in your lab and sequence in ours.  GTAC@MGI can include partial runs on a NovaSeq 6000 s4 to accommodate your project.

Library Preparation Options

For each sequencing option we have corresponding library preparation choices. 

Whole Genome

  • KAPA Hyper PCR-Free  with an automated process
  • SWIFT with a manual process and low input to start.
  • SWIFT WGBS  with a manual process and low input to start.
  • IDT with Unique Molecular Identifiers (UMIs) and a manual process with low input to start.

The above options include initial DNA quantitation check with the Qubit.

Whole Exome

  • KAPA Hyper amplified with an automated process

The above option includes initial DNA quantitation check with the Qubit.


This preparation includes initial DNA quality and quantitation check with the Qubit and Tapestation or the Bioanalyzer.