Single Cell & Spatial

Study your single cell project through next-generation sequencing or quantitatively measuring your gene expression.

Next-Generation Sequencing

Next-Generation Sequencing captures the process of massively parallel sequencing of millions of fragments simultaneously per Illumina run. This high-throughput process translates into sequencing hundreds to thousands of genes at one time while allowing for the review of individual cells.  For single-cell, there are a lot of 10x Genomics options to focus on your research.

  • 10x Genomics Single Nuclei ATACseq
  • 10x Genomics Multiome RNAseq and ATACseq
  • 10X Genomics 3′ GEX Single-Cell Library
  • 10X Genomics 3′ Feature Barcoding (Cell Surface Protein, CRISPR)
  • 10X Genomics 3′ HT Single-Cell GEX Library
  • 10X Genomics 3′ HT Feature Barcoding (Cell Surface Protein, CRISPR)
  • 10X Genomics 5′ GEX Single-Cell Library
  • 10X Genomics 5′ GEX V(D)J enrichment
  • 10X Genomics 5′ GEX Feature Barcoding (Cell Surface Protein, CRISPR)
  • 10x Genomics SinglePlex Fixed/Flex Assay
  • 10x Genomics SinglePlex Fixed/Flex Assay with Feature Barcoding (Cell Surface Protein)
  • 10x Genomics MultiPlex Fixed/Flex Assay (2-4 samples)
  • 10x Genomics MultiPlex Fixed/Flex Assay (5-16 samples)
  • 10x Visium Spatial – FFPE Blocks
  • FACSseq Single-Cell RNAseq
  • SMARTSeq2 Single-Cell RNAseq – 96 well plate format

Single Cell Analysis

GTAC@MGI provides a variety of files based on the pipeline used for your research. Single-cell post-processing deliverables that are automatically provided for your convenience are noted below.

  • SMRT-seq (FACS-seq)-FASTQ files, BAM files, count tables, cell clusters, differentially expressed genes
  • 10x Cell Ranger scRNA-seq-FASTQ files, Cell Ranger output: *, matrix files
  • 10x Cell Ranger ATAC-seq- FASTQ files, Cell Ranger ATAC output: *, matrix files
  • 10x Cell Ranger Feature Barcodes- FASTQ files, Cell Ranger output: *, matrix files
  • 10x Cell RangerVDJ analysis-FASTQ files, Cell Ranger output: *, matrix files
  • 10x Cell Ranger Multiomic analysis- FASTQ files, Cell Ranger ARC output: *, matrix files
  • 10x Space Ranger analysis-FASTQ files, Space Ranger output: *, matrix files

Coming Soon

  • 10x Genomics FFPE on CytAssist for Visium Spatial
  • 10x Genomics Fresh Frozen on CytAssist for Visium Spatial
  • 10x Genomics BEAM Feature Technology (Barcode Enabled Antigen Mapping)
  • 10x Genomics Xenium
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