Next-Generation Sequencing

Next-Generation Sequencing captures the process of massively parallel sequencing of millions of fragments simultaneously per Illumina run. This high-throughput process translates into sequencing hundreds to thousands of genes at one time while allowing for the review of individual cells.  For single-cell, there are a lot of 10x Genomics options to focus on your research.

• 10x Genomics Single Nuclei ATACseq
• 10X Genomics 3′ v3 GEX Single-Cell Library
• 10X Genomics 3′ v3  GEX Library with Feature Barcoding
• 10X Genomics 5′ GEX Single-Cell Library
• 10X Genomics 5′ GEX Single-Cell Library one V(D)J enrichment
• 10X Genomics 5′ GEX Single-Cell Library with two V(D)J enrichments
• 10X Genomics 5′ GEX Single-Cell Library with Feature Barcoding
• 10X Genomics 5′ GEX Single-Cell Library with T cell Enrichment and Feature Barcoding
• 10X Genomics 5′  GEX Single-Cell Library with T cell and B cell Enrichment and Feature Barcoding
• FACSseq Single-Cell RNAseq

Single Cell Analysis

GTAC@MGI provides a variety of files based on the pipeline used for your research. Single-cell post-processing deliverables that are automatically provided for your convenience are noted below.

• SMRT-seq (FACS-seq)-FASTQ files, BAM files, count tables, cell clusters, differentially expressed genes
• 10x Cell Ranger scRNA-seq-FASTQ files, Cell Ranger output: BAM files, matrix files
• 10x Cell Ranger ATAC-seq- FASTQ files, Cell ranger ATAC output: BAM files, matrix files
• 10x Cell Ranger Feature Barcodes- FASTQ files, Cell Ranger output: BAM files, matrix files
• 10x Cell RangerVDJ analysis-FASTQ files, Cell Ranger output: BAM files
• 10x Cell Ranger Multiomic analysis- FASTQ files, Cell Ranger ARC output: BAM files