GTAC@MGI provides quantitative PCR and amplicon generation to assist your studies If your research needs targeted sequencing, SNP genotyping, or gene expression profiling, we can assist.
Most qPCR services leverage the use of Taqman assays, but GTAC@MGI can also provide services utilizing EvaGreen dye and compatible primers. For low to moderate throughput, projects can be completed via BioRad CFX. For high throughput projects, we utilize the Fluidigm BioMark™ HD System. Several formats are available on the Fluidigm platform, utilizing their integrated fluidic circuits (IFCs). Primary formats (samples/assays):
Known regions of interest are captured with Amplicon sequencing. This highly targeted approach enables applications for discovering, validating, and screening genetic variants in a rapid and efficient manner. This is accomplished by the multiplexing capabilities of the Fluidigm Juno and Access Array systems. These applications are economical for large sample number projects, typically 1K samples or larger