GTAC@MGI provides comprehensive guidelines for submitting samples across a wide array of platforms. Please review the specific requirements for your project to ensure high-quality data generation and efficient processing of your short-read, long-read, single cell, spatial, high-plex proteomics, high-throughput qPCR, and microarray samples.
Prior to sample receipt, complete a Service Request
here.
A printout of the Service Request must be included with the material.
Tubes: Strip tubes and screw cap tubes will not be accepted.
Sample QC
Sample (Nucleic Acid) QC Only sample submission guidelines.
Sample (Nucleic Acid) QC Only
Tubes: Prepare samples in clearly labeled and well-organized 1.7 mL flip-cap microcentrifuge tubes.
Plates: For projects with 48 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange samples vertically by column (A1, B1, C1, and so on).
Volume: 3µl or more per sample. If requesting both Qubit and Agilent services, 5µl or more.
Concentration: Must be within range for the selected assay type.
RNA
RNA sample submission guidelines and RNA seq input recommendations.
RNA Sample Submission Guidelines
Tubes: Prepare samples in clearly labeled and well-organized 1.7 mL flip-cap microcentrifuge tubes.
Plates: For projects with 48 or more samples, prepare samples in clearly labeled, securely sealed 96-well full-skirted PCR v-bottom plates. Arrange samples vertically by column (A1, B1, C1, and so on).
Solvent: RNase free water.
RNA should be DNase treated and purified.
PolyA selection
Input Quantity: 1 to 5ug
Volume: more than 10µl
Concentration: 20ng/µl or more
Quality: RIN greater than 8, DNA-free
RiboErase (human / mouse / rat)
Input Quantity: 500ng to 1ug
Volume: more than 10µl
Concentration: 50ng/µl or more
Quality: Compatible with degraded RNA if all samples degraded similarly, DNA-free
FastSelect (human / mouse / rat)
Input Quantity: 500ng to 1ug
Volume: more than 10µl
Concentration: 50ng/µl or more
Quality: Compatible with degraded RNA if all samples degraded similarly, DNA-free
FastSelect with rRNA and globin depletion (whole blood)
Input Quantity: 500ng to 1ug
Volume: more than 10µl
Concentration: 56ng/µl or more
Quality: Compatible with degraded RNA if all samples degraded similarly, DNA-free
Watchmaker with rRNA and globin depletion (human / mouse / rat)
Input Quantity: 100ng to 1ug
Volume: more than 10µl
Concentration: 6ng/µl or more
Quality: Compatible with degraded RNA if all samples degraded similarly, DNA-free
Takara SMARTseq mRNA (low input)
Input Quantity: 10ng or more
Volume: more than 10µl
Concentration: 1.1ng/µl or more
Quality: RIN greater than 8, DNA-free
Sigma SeqPlex
Quantity: 10ng or more (FFPE samples: 50ng)
Volume: more than 10µl
Concentration: 1.2ng/µl or more (FFPE samples: 6ng/µl or more)
Quality: Compatible with degraded RNA, often utilized for FFPE, DNA-free
DNA
DNA sample submission guidelines, DNA input recommendations, ChIP seq, and amplicon seq.
DNA Sample Submission Guidelines
Tubes: Prepare samples in clearly labeled and well-organized 2D matrix tubes (preferred) or 1.7 mL flip-cap microcentrifuge tubes.
Solvent: Appropriate elution buffer (for example, 10mM Tris HCl).
Kapa Hyper PCR free
Input Quantity: 600ng to 1ug or more
Volume: 20µl or more
Concentration: 20 to 100ng/µl
Increased data targets may require additional starting material.
Kapa Hyper amplified
Input Quantity: 100ng to 500ng or more
Volume: 20µl or more
Concentration: 10 to 100ng/µl
Increased data targets may require additional starting material.
IDT cfDNA FFPE WGS with PCR
Input Quantity: 5 to 100ng or more
Volume: 20µl or more
Concentration: 2 to 100ng/µl
NEB EM seq Methyl Seq
Input Quantity: 10 to 200ng or more
Volume: 20µl or more
Concentration: 4 to 100ng/µl
IDT WGBS Methyl Seq
Input Quantity: 100 to 200ng or more
Volume: 20µl or more
Concentration: 4 to 100ng/µl
ChIP seq
Solvent: DNase free water
Fragment to 150 to 500bp prior to IP
Input Quantity: 10ng
Volume: more than 10 to 30µl
Concentration: 0.33ng/µl or more
Amplicon seq
Discuss with GTAC@MGI prior to request to confirm suitability.
Pool or concatemerized amplicons will be uniquely dual indexed.
Minimum sequencing for NovaSeq X shared lane: 150M read pairs
Prepared Libraries
Prepared libraries sequencing only, and prepared libraries plus final library QC.
Tubes: Prepare libraries in clearly labeled and well-organized 2D matrix tubes (preferred) or 1.7 mL flip-cap microcentrifuge tubes. If submitting 30 tubes or more, request 2D tubes from GTAC@MGI.
Indicate uneven pooling ratios if needed in the sample manifest.
Volume: 30µl or more
Concentration: 5 to 30nM
Additional qPCR charges apply if libraries or pools are outside this range.
When requesting a full 1.5B or 10B lane, submit 300µl or more at 5nM or more.
When requesting a full 25B lane, submit 480µl or more at 5nM or more.
Prepared Libraries (plus Final Library QC)
Tubes: Prepare libraries in clearly labeled and well-organized 1.7 mL flip-cap microcentrifuge tubes or 96-well PCR plates.
Indicate uneven pooling ratios if needed in the sample manifest.
Concentration: 5 to 30nM
Volume: individual libraries 10 to 20µl; pool of libraries 30µl or more
Low volume or concentration libraries may be rejected if we cannot make a balanced pool to meet minimum of 5nM in 30µl.
Single Cell and Spatial
Single cell and spatial sample submission guidelines.
10x Genomics
Tubes: Prepare samples in clearly labeled and well-organized 1.7 mL flip-cap microcentrifuge tubes.
Date and time of delivery must be scheduled with 10x Single Cell Core prior to sample delivery. Samples are processed immediately upon receipt.
