Single Cell & Spatial

Study your single cell project through next-generation sequencing or quantitatively measuring your gene expression.

Next-Generation Sequencing/ Spatial

Next-Generation Sequencing captures the process of massively parallel sequencing of millions of fragments simultaneously per Illumina run. This high-throughput process translates into sequencing hundreds to thousands of genes at one time while allowing for the review of individual cells.  For single-cell, there are a lot of 10x Genomics options to focus on your research.

  • 10x Genomics Single Nuclei ATACseq
  • 10x Genomics Multiome RNAseq and ATACseq
  • 10x Genomics 3′ GEM-X GEX Single-Cell Library
  • 10x Genomics 3′ GEM-X Feature Barcoding (Cell Surface Protein)
  • 10x Genomics 5′ GEM-X GEX Single-Cell Library
  • 10x Genomics 5′ GEM-X GEX V(D)J enrichment
  • 10x Genomics 5′ GEM-X GEX Feature Barcoding (Cell Surface Protein)
  • 10x Genomics 3’ or 5’ OCM (On Chip Multiplexing)
    • Feature Barcoding (Cell Surface Protein)
    • 5’ V(D)J
  • 10x Genomics SinglePlex GEM-X Flex
    • Feature Barcoding (Cell Surface Protein)
  • 10x Genomics MultiPlex GEM-X Flex/(2-4 samples) or (5-16 samples)
    • Feature Barcoding (Cell Surface Protein)
  • 10x Visium HD Spatial – FFPE or Fresh Frozen
    • 6.5×6.5mm only
  • 10x Xenium V1 Spatial – FFPE or Fresh Frozen
    • Advanced Cell Segmentation upon request
  • 10x Xenium Prime – 5K panel – FFPE or Fresh Frozen

Single Cell Analysis

GTAC@MGI provides a variety of files based on the pipeline used for your research. Single-cell post-processing deliverables that are automatically provided for your convenience are noted below.

  • SMRT-seq (FACS-seq)-FASTQ files, BAM files, count tables, cell clusters, differentially expressed genes
  • 10x Cell Ranger scRNA-seq-FASTQ files, Cell Ranger output: *, matrix files
  • 10x Cell Ranger ATAC-seq- FASTQ files, Cell Ranger ATAC output: *, matrix files
  • 10x Cell Ranger Feature Barcodes- FASTQ files, Cell Ranger output: *, matrix files
  • 10x Cell RangerVDJ analysis-FASTQ files, Cell Ranger output: *, matrix files
  • 10x Cell Ranger Multiomic analysis- FASTQ files, Cell Ranger ARC output: *, matrix files
  • 10x Space Ranger analysis-FASTQ files, Space Ranger output: *, matrix files
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