Bulk RNA-Seq at GTAC@MGI supports transcriptome profiling for differential expression studies using Illumina® platforms, with library protocol selection guided by RNA quality, input amount, and experimental goals. Libraries are uniquely dual indexed for multiplexing, with sequencing depth matched to the study design.
PolyA selection enriches for polyadenylated transcripts and is commonly used for eukaryotic gene expression profiling. Library protocol is selected based on RNA integrity, input amount, and experimental goals.
Request a quote General inquiryRibosomal depletion allows for efficient detection of functionally relevant coding as well as non-coding transcripts through removal of highly abundant rRNA species
Blood derived RNA often contains abundant globin transcripts that can dominate sequencing. Ribosomal and globin depletion reduces background and increases effective coverage for informative features.
Low input workflows support studies where RNA quantity is limiting. Selection depends on input amount, sample type, and quality.
Library QC available upon request, including Qubit quantification, Agilent Bioanalyzer traces, and pooling prior to sequencing. For expedited services, libraries can be submitted directly for qPCR quantification and sequencing only.
Bulk RNA-Seq performance is driven by input material and selection of appropriate library protocol. The comparison below summarizes practical tradeoffs between common approaches used for gene expression profiling and transcriptome characterization, including PolyA selection, ribosomal depletion, and low input workflows. Selection is typically refined using RNA quality metrics, sample source, and study design.
| Protocol | What it captures | Works best when | Key limitations |
|---|---|---|---|
| PolyA selection | Polyadenylated mRNA transcripts | RNA is high quality and the focus is coding gene expression | Requires higher input mass, biased performance with degraded RNA |
| Ribosomal depletion | Coding and noncoding transcripts from total RNA | RNA is partially degraded or whole transcriptome coverage is needed | Limited species compatibility, may require increased sequencing depth compared with PolyA selection |
| Ribosomal and globin depletion | Coding and noncoding transcripts from total RNA | Samples are whole blood derived or globin transcripts dominate | Limited species compatibility, may require increased sequencing depth compared with PolyA selection |
| Low input workflows | Transcriptome from limited RNA amounts | Sample quantity is limiting | Caveats could include 3′ bias, high rRNA content, or other amplification bias dependent on sample quality and quantity |
High throughput short read sequencing system optimized for large scale transcriptomic studies.
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High accuracy short read sequencing system powered by avidity chemistry for flexible transcriptomic workflows.
