Sequencing

Whether you are a novice or an expert in NGS sequencing technologies, GTAC can help you design a study, prepare DNA or RNA libraries, sequence samples, and analyze the data.


How to Get Started

To obtain a quote or discuss a project, contact us via our project initiation form.  

If you would like to submit a sample, please review our sample submission guidelines (table format) and contact us for submission instructions.  Below you will find more information about our sequencing services: library preparation, sequencing, and analyses.

Library Preparation

GTAC offers a wide selection of library prep types for Illumina sequencing.  Below is a list of prep types that we offer.  For further description of these prep types and/or a consultation with our team to discuss experimental design, please contact gtac@genetics.wustl.edu

For CAP/CLIA accredited services please visit Genomics and Pathology Services at http://wugps.wustl.edu/

Sequencing

GTAC operates four Illumina HiSeq 2500 sequencing instruments, one HiSeq 3000 instrument and two MiSeq instruments. The HiSeq is Illumina’s most up-to-date sequencer and workhorse for NGS sequencing. The HiSeq 2500 can run two flowcells simultaneously, and the HiSeq 3000 runs one flowcell at a time.  DNA fragments can be read from one or two ends to produced “paired end” reads

For the HiSeq 2500, we currently offer 2x101 paired end and 1x50 single read sequencing runs. Both run types are standardly offered in rapid mode, where each flowcell has two lanes, and each lane averages 150 million reads.  Other run types can be accommodated if you can fill
at least two lanes at a time; that is, fill a flow cell.

For the HiSeq 3000, we offer 1x50 single read, 2x75 paired end, and 2x150 paired end sequencing runs.  Each flowcell has 8 lanes, and each lane averages 312 million reads.  Other run types can be accomodated if you can fill all eight lanes at a time; that is, fill a flow cell.

GTAC also operates two Illumina MiSeq instruments. The MiSeq runs one single-lane flow-cell per run. Each run is capable of producing 12-15 million reads. We currently offer 2x150 paired end and 2x250 paired end sequencing runs in version 2 chemistry. We can also run the nano and micro flow-cells, if interested please contact us.
 
More information about the sequencing instruments can be found here: HiSeq 2500 HiSeq 3000 Illumina-MiSeq

Analysis

The sequence analysis group performs a number of analyses, which are included in the price of sequencing, to assist customers with their research needs.  The goal of our analyses is to empower investigators by making next generation sequencing accessible to all users.  We do this by performing complex and time consuming steps on a large computational cluster so researchers can investigate specific hypotheses on their personal computers.
 

Full Analysis Pipelines:
 
Additionally, we perform some basic manipulations that allow more advanced users flexibility with downstream analyses.
 
Partial Analyses:
 
Fully Supported Organisms:
 Human  Homo sapien
 Mouse  Mus musculus
 Zebrafish  Danio rerio
 Fruit fly  Drosophila melanogaster
 Nematode  Caenorhabditis elegans
 Yeast  Saccharomyces cerevisiae
 E. coli  Escherichia coli
 
Others Organisms *:
 Chicken  Gallus gallus
 Arabidopsis  Arabidopsis thaliana
 C. briggsae  Caenarhabditis briggsae
 Chlamydamonas  Chlamydamonas reihardtii
 Plasmodium  Plasmodium falciparum
 Cryptococcus  Cryptococcus neoformans
* For analyses on organisms not listed, please inquire to gtac-bioinformatics@googlegroups.com
* For analyses on other organisms, please inquire to [....]Full Analysis Pipelines:
Exome – targeted capture analysis
Genome analysis
RNA-Seq
ChIP-Seq
 
 
Additionally, we perform some basic manipulations that allow more advanced users flexibility with downstream analyses.
 
Partial Analyses:
Demultiplexing
Sequence alignment
 
 
Fully Supported Organisms:
Human Homo sapien
Mouse Mus musculus
Zebrafish Danio rerio
Fruit fly Drosophila melanogaster
Nematode Caenorhabditis elegans
Yeast Saccharomyces cerevisiae
E. coli Escherichia coli
 
Others Organisms:
Chicken Gallus gallus
Arabidopsis Arabidopsis thaliana
C. briggsae Caenarhabditis briggsae
Chlamydamonas Chlamydamonas reihardtii
Plasmodium Plasmodium falciparum
Cryptococcus Cryptococcus neoformans
 
* For analyses on other organisms, please inquire to [....]

 

Full Analysis Pipelines:
Exome – targeted capture analysis
Genome analysis
RNA-Seq
ChIP-Seq
 
 
Additionally, we perform some basic manipulations that allow more advanced users flexibility with downstream analyses.
 
Partial Analyses:
Demultiplexing
Sequence alignment
 
 
Fully Supported Organisms:
Human Homo sapien
Mouse Mus musculus
Zebrafish Danio rerio
Fruit fly Drosophila melanogaster
Nematode Caenorhabditis elegans
Yeast Saccharomyces cerevisiae
E. coli Escherichia coli
 
Others Organisms:
Chicken Gallus gallus
Arabidopsis Arabidopsis thaliana
C. briggsae Caenarhabditis briggsae
Chlamydamonas Chlamydamonas reihardtii
Plasmodium Plasmodium falciparum
Cryptococcus Cryptococcus neoformans
 
* For analyses on other organisms, please inquire to [....]