GENOME TECHNOLOGY ACCESS CENTER • DEPARTMENT OF GENETICS • WASHINGTON UNIVERSITY SCHOOL OF MEDICINE

Multi-Domain 16s

MVRSION was recently developed for the purpose of using 16S to define metabiomic bacterial populations from human, animal, or environmental sources. The suite is a methodology that simultaneously leverages eight 16S hyper-variable regions to characterize either host or environmental metagenomes. We use Fluidigm Access Array or Juno to economically prep the eight regions. These are all sequenced and analyzed in parallel, using the MVRSION computational protocol. The underlying database is based off of Silva release 123 which we curated to remove all sequences which do not contain a bacterial genus/species. MVRSION will call a species or, if no species is found, a genus is called from a separate genus database. We will soon release a version of the database which includes curated versions of both Silva and RDP databases.

Want to learn more?

Feel free to check out our 16s publication at  https://www.ncbi.nlm.nih.gov/pubmed/30273610 to learn more…